We prepared glass slides to view ATTO molecules fluoresce and to measure their fluorescent lifetime. The molecules have a tendency to clump together in water on a glass slide. To get the cover-slips to be hydrophobic, it was coated with a hydrophobic chemical, OTS(octadecytrichloro-silane). The cover-slips were plasma cleaned and then washed in an ultra-sonicator bath first with cloroform, then in ethanol and finally with water. It takes a long time for the water to evaporate or dry from the slides. Next, we try to dilute the dye in ethanol which evaporates faster than water. Then it was time to go for lunch.
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