Tuesday, July 6, 2010
July 6th AM
Today, we look at pre-cancerous cells from the human esophagus. They are from the CPD, CPA, EPC cell lines. The cells were dyed with fluorescent dyes that stain the nucleoli yellow and DNA blue. Cells were put on a petri-dish heater to heat them to body temperature of 37 degrees Celsius. The cells survive longer at 37 degrees than at 25 degrees or room temperature. Cells can survive up to 6 hrs. at 37 degrees compared to 2 hrs. at 25 degrees. The cells were scan with the cantilever tip in the oscillating mode. A picture of a cell with a big blue nucleus and a yellow nucleolus was seen. The cells were flattened on petri-dish and each squamous cell is about 20-25 micron.They zoom in on the nucleus of a cell to take another scan. Then, it is time for lunch.
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